SCIENCE & TECH
WE ARE USING CRISPR
CRISPR is an abbreviation of
Clustered Regularly Interspaced Short Palindromic Repeats
What is CRISPR?
CRISPR genome editing technology. In the field of genome engineering, the term “CRISPR” is often used to refer to the various CRISPR-Cas9 and -CPF1 systems that can be programmed to target specific stretches of the genetic code and to edit DNA at precise locations.
How does CRISPR-Cpf1 differ from CRISPR-Cas9?
Cpf1 cuts DNA in a different manner than Cas9. When the Cas9 complex cuts DNA, it cuts both strands at the same place, leaving ‘blunt ends’ that often undergo mutations as they are rejoined. With the Cpf1, the cuts in the two strands are offset, leaving short overhangs on the exposed ends. This is expected to help with precise insertion, allowing to integrate a piece of DNA more efficiently and accurately. The Cpf1 enzyme is also smaller than the standard Cas9, making it easier to deliver into cells and tissues.
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